Process design for enantioselective syntheses of amines based on the use of chemocatalysts and transaminases

Fellow: ESR11 Host Institution: Chair of Organic Chemistry Bielefeld University -Germany Secondments: Entrechem -Spain Supervisors: H. Gröger & A. Hummel (Uni Bielefeld), F. Moris & J. Sabin (Entrechem) This PhD will focus on the combination of palladium-catalyzed Suzuki-cross coupling reactions with biocatalytic transaminations of ketones towards one-pot processes for the enantioselective syntheses of a range […]

Widening the substrate scope of transaminases by protein engineering

Fellow: ESR10 Host Institution: Institute of Molecular Biotechnology Graz University of Technology- Austria Secondments: DSM-The Netherlands Supervisors: H. Schwab (TU Graz), K. Steiner (acib), M. Schürmann (DSM) This PhD will focus on analyzing previously identified transaminase structures for restrains for binding of functionalized bulky or bulky-bulky ketones. It also focuses on widening the substrate scope […]

Upscaling of ω-transaminase catalysed cascade reactions for high-value chemicals production

Fellow: ESR9 Host Institution: School of Biotechnology Kunglinga Tekniska Högskolan (KTH)- Sweden Secondments: Entrechem-Spain, Enzymicals-Germany Supervisors: P. Berglund (KTH), F.Moris & J. Sabin (Entrechem), R. Wardenga (Enzymicals) This PhD will focus on the scale-up and optimization experiments for production of high-value compounds. The ESR is expected to optimize reaction protocol to allow production of industrially […]

Transaminase-catalyzed de-racemization cascade reactions

Fellow: ESR8 Host Institutions: Saromics Biostructures-Sweden Secondments: Royal Institute of Technology KTH-Sweden, Viazym-Netherlands Supervisors: B. Walse, D. Logan (Saromics), L. van Langen (Viazym), P. Berglund (KTH) This PhD will focus on the design and produce new stable enzyme variants suitable for cascade reactions in organic or biphasic media by protein engineering, X-ray crystallography studies, bioinformatic […]

Combining amine transaminase (ATA) and keto reductase (KRED) for 1-amino-cyclohexane-4-ol synthesis.

Fellow: ESR7 Host Institutions: Junior Professorship for Protein Biochemistry, Ernst Moritz Arndt University Greifswald Supervisors: M. Höhne (University Greifswald), F. Moris, J. Sabin (Entrechem), R. Wardenga (Enzymicals) The research project “Combining amine transaminase (ATA) and keto reductase (KRED) for 1-amino-cyclohexane-4-ol synthesis” presupposes obtaining of optically pure 1-amino-cyclohexane-4-ol, applying enzymatic transamination and ketoreduction.1-amino-cyclohexane-4-ol is a valuable […]

Novel amino donors for efficient equilibrium shift in transaminations

Fellow: ESR6 Host Institutions: Dept. of Organic Chemistry, University Oviedo, and Entrechem Supervisors: F. Rebolledo (University Oviedo), F. Moris, J. Sabin (Entrechem), R. Wardenga (Enzymicals) This PhD will focus on the identification of suitable achiral amino donors for an efficient equilibrium shift in amine-TA catalyzed reactions. This will lead to the assembly of a two-enzyme […]

Synthesis of enantiopure 1,3/1,4- aminoalcohols from prochiral ketoacids

Fellow: ESR5 Host Institution: Junior Professorship Microbial Biotechnology, Ruhr University Bochum Supervisors: R. Kourist (Ruhr University Bochum), B. Walse, D. Logan (Saromics), F. Moris, J. Sabin (Entrechem) This PhD will focus on the development of novel enzyme cascades for the synthesis of enantiopure hydroxy amino acids and amino alcohols, valuable building blocks for the pharmaceutical […]

Synthesis of enantiopure 1,2-aminoalcohols from alkenes

Fellow: ESR3 Host Institution: Institute of Microbiology, TU Braunschweig Supervisors: A. Schallmey (TU Braunschweig), F. Hollmann (TU Delft), R. Wardenga (Enzymicals) This PhD candidate will focus on the identification of suitable enzyme combinations (P450s or lipases in combination with halohydrin dehalogenases) for the synthesis of 1,2-azidoalcohol targets from respective alkenes in combination with a chemical […]

Synthesis of enantiopure 1,2-aminoalcohols from α-haloketones

Fellow: ESR2 Host Institution: Enzymicals Supervisors: R. Wardenga (Enzymicals), F. Hollmann (TU Delft) This PhD will focus on the optimization of one pot synthesis sequence and enzyme production. The ESR is expected to produce Enantio- and/or diastereomerically pure 1,2-aminoalcohols at preparative scale. Objectives 1. Optimization of the one pot synthesis sequence (reaction conditions, enzyme formulation) […]