Synthesis of enantiopure 1,2-aminoalcohols from alkenes
This PhD candidate will focus on the identification of suitable enzyme combinations (P450s or lipases in combination with halohydrin dehalogenases) for the synthesis of 1,2-azidoalcohol targets from respective alkenes in combination with a chemical reduction to finally obtain 1,2-aminoalcohols. Thus, the main objective of the project will be the assembly of a new chemo-enzymatic cascade towards 1,2-aminoalcohols. This might also involve protein engineering of one of the enzymes to improve specific enzyme properties. In a secondment to the company Enzymicals, the ESR will further focus on the upscaling of the cascade to produce the respective enantio- and/or diastereomerically pure aminoalcohols at preparative scale.
1. Identification of suitable enzyme combinations (CYP or lipase with HHDH) for the synthesis of 1,2-azidoalcohol targets
2. Assembly of a new two-enzyme cascade (whole cells or isolated enzymes) in combination with a chemical catalyst
3. Upscaling of the cascade and proof-of-principle of noradrenaline synthesis in 50 g-scale
4. Optimization of enzyme production in 50 L-scale