Synthesis of enantiopure 1,2-aminoalcohols from alkenes

Fellow: ESR3
Host Institution: Institute of Microbiology, TU Braunschweig
Supervisors: A. Schallmey (TU Braunschweig), F. Hollmann (TU Delft), R. Wardenga (Enzymicals)

This PhD candidate will focus on the identification of suitable enzyme combinations (P450s or lipases in combination with halohydrin dehalogenases) for the synthesis of 1,2-azidoalcohol targets from respective alkenes in combination with a chemical reduction to finally obtain 1,2-aminoalcohols. Thus, the main objective of the project will be the assembly of a new chemo-enzymatic cascade towards 1,2-aminoalcohols. This might also involve protein engineering of one of the enzymes to improve specific enzyme properties. In a secondment to the company Enzymicals, the ESR will further focus on the upscaling of the cascade to produce the respective enantio- and/or diastereomerically pure aminoalcohols at preparative scale.

Objectives
1. Identification of suitable enzyme combinations (CYP or lipase with HHDH) for the synthesis of 1,2-azidoalcohol targets
2. Assembly of a new two-enzyme cascade (whole cells or isolated enzymes) in combination with a chemical catalyst
3. Upscaling of the cascade and proof-of-principle of noradrenaline synthesis in 50 g-scale
4. Optimization of enzyme production in 50 L-scale

The Biocascades Project is a joint collaboration among the following university and industrial partners