Novel amino donors for efficient equilibrium shift in transaminations

Fellow: ESR6
Host Institutions: Dept. of Organic Chemistry, University Oviedo, and Entrechem
Supervisors: F. Rebolledo (University Oviedo), F. Moris, J. Sabin (Entrechem), R. Wardenga (Enzymicals)

This PhD will focus on the identification of suitable achiral amino donors for an efficient equilibrium shift in amine-TA catalyzed reactions. This will lead to the assembly of a two-enzyme cascade with in situ cofactor regeneration for equilibrium shift. The ESR is expected to broaden the scope of the enzymatic transamination reactions to new amino-donors and acceptors and to produce enantiomerically and diastereomerically pure amines, diamines and AAs as products of these reactions.

Objectives
1. Identification of suitable achiral amino donors for an efficient equilibrium shift in amine-TA catalyzed reactions.
2. Assembly of a two-enzyme cascade with in situ cofactor regeneration for equilibrium shift.
3. Application of the identified amino-donor / developed cascade for the synthesis of commercially relevant products, e.g., amino alcohols, diamines and bulky amines.
4. Proof-of-principle in 500 g-scale for synthesis of 4-hydroxycyclohexylamine (precursor for derenofylline, and lombuvir), “tech transfer package” for transfer to industrial partner

The Biocascades Project is a joint collaboration among the following university and industrial partners